Genetic diversity of eight maize (Zea mays) cultivars from East Nusa Tenggara (Indonesia) based on inter simple sequence repeat markers

Abstract. Yani A, Chasani AR, Daryono BS. 2022. Genetic diversity of eight maize Zea mays L. cultivars from East Nusa Tenggara based on inter simple sequence repeat markers. Biodiversitas 23: 4124-4130. (NTT) is one Indonesia's island that has many local cultivars. However, the existence this corn threatened by presence high-yielding seeds. Therefore, characterization using molecular marker needed to conserve germplasm. This study aims determine genetic (Zea maysL.) ISSR research used (Watar Ohaq, Watar Puluq, Wata Bura, Kuma, Bunga, Pena Muti, Boti Pulut, and Latun Wara). Young leaves were for DNA isolation. Total genomic was isolated Genomic Mini Kit Plant (Geneaid). then amplified 5 markers (ISSR-807, ISSR-808, UBC-809, UBC-811, UBC-834). band profiles scored or absence bands. variability analyzed UPGMA method MVSP ver application. 3.1. Local NTT high (similarity index between 0.29-0.65). separated into two main clusters at a similarity 0.43 grouping pattern not influenced geographic location origin.